KMID : 0364820140500030254
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Korean Journal of Microbiology 2014 Volume.50 No. 3 p.254 ~ p.260
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Isolation and Characterization of Sulfate- and Sulfur-reducing Bacteria from Woopo Wetland, Sunchun Bay, and Tidal Flat of Yellow Sea
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Kim So-Jeong
Min Ui-Gi Hong Hee-Ji Kim Jong-Geol Jung Man-Young Cha In-Tae Rhee Sung-Keun
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Abstract
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Sulfur compound includes major electron acceptors for anaerobic respiration. In this study, cultivation-based study
on sulfate- and sulfur-reducing bacteria of various wetlands of Korea was attempted. To isolate sulfate- and sulfur-reducing bacteria, anaerobic roll tube method was used to obtain typical black colonies of sulfate- and sulfur-reducing
bacteria. Total 11 strains obtained were tentatively identified based on comparative 16S rDNA similarity and
physiological property analysis. All sulfate-reducing bacteria (8 strains) belonged to genus Desulfovibrio with >99%
16S rDNA similarities. Three sulfur reducing bacteria were also isolated: two and one isolates were affiliated with
Sulfurospirillum and Desulfitobacterium, respectively. These sulfate- and sulfur-reducing bacteria were able to
utilize lactate and pyruvate and sulfite and thiosulfate as common electron donors and electron acceptors,
respectively. This case study will provide fundamental information for obtaining useful indigenous sulfate- and
sulfur-reducing bacteria from Korean wetlands employing various combinations of cultivation conditions.
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KEYWORD
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Desulfovibrio, anaerobic bacteria, sulfate-reducing bacteria, sulfur-reducing bacteria
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